Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-04-12 |
Detector | ADSC QUANTUM |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.507, 81.833, 98.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.600 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.22800 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.048 | 0.318 |
Number of reflections | 67818 | |
<I/σ(I)> | 40.4 | |
Completeness [%] | 93.0 | 61.4 |
Redundancy | 8.9 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.4 | 18 * | 29% PEG 4000, 100MM TRIS, 175 LISO4, 10MM DMF, pH 8.40 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 29 (%) | |
3 | 1 | reservoir | Tris | 100 (mM) | |
4 | 1 | reservoir | 160-185 (mM) | ||
5 | 1 | reservoir | dimethylformamide | 8-12 (%) |