1C7G

TYROSINE PHENOL-LYASE FROM ERWINIA HERBICOLA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1998-05-01
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	163.490, 113.040, 101.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.1862
Rwork	0.186
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tpl
RMSD bond length	0.007
RMSD bond angle	1.442
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR
Refinement software	X-PLOR (3.85)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	2.079
High resolution limit [Å]	2.007	2.007
Number of reflections	113432
<I/σ(I)>	1.34
Completeness [%]	90.3	53.7
Redundancy	4.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.2		30% PEG6000, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, PH 6.2