1C6V
SIV INTEGRASE (CATALYTIC DOMAIN + DNA BIDING DOMAIN COMPRISING RESIDUES 50-293) MUTANT WITH PHE 185 REPLACED BY HIS (F185H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.570, 100.000, 150.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.000 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.36200 |
| Structure solution method | SIR + MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ITG AND 1IHV |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.113 | 0.608 |
| Total number of observations | 309679 * | |
| Number of reflections | 22129 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 92.5 | 66.8 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.7 | 0.1 M MES, PH=5.7, PEG6K 8%, 15% DIOXANE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Mes | 0.1 (M) | |
| 2 | 1 | 1 | PEG6000 | 8 (%(v/v)) | |
| 3 | 1 | 1 | dioxane | 1.5 (%(v/v)) |
