1C5K
THE STRUCTURE OF TOLB, AN ESSENTIAL COMPONENT OF THE TOL-DEPENDENT TRANSLOCATION SYSTEM AND ITS INTERACTIONS WITH THE TRANSLOCATION DOMAIN OF COLICIN E9
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91, 1.07, 1.386, 1.387 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.580, 40.190, 77.660 |
| Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27100 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 17.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 * | 0.176 * |
| Number of reflections | 59093 | |
| Completeness [%] | 98.4 | 97.4 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 10 (mM) | |
| 3 | 1 | 2 | PEG8000 | 18 (%(w/v)) | |
| 4 | 1 | 2 | MES | 100 (mM) |
