1C3X

PURINE NUCLEOSIDE PHOSPHORYLASE FROM CELLULOMONAS SP. IN COMPLEX WITH 8-IODO-GUANINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	63.319, 108.340, 117.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.000 - 2.400
R-factor	0.2
Rwork	0.200
R-free	0.25000 *
RMSD bond length	0.015 *
RMSD bond angle	2.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.420
High resolution limit [Å]	2.250 *	2.250
Rmerge	0.081	0.444
Number of reflections	35723
<I/σ(I)>	9.3
Completeness [%]	90.0	64
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *	298	PEG 4000, CACODYLATE, CA-ACETATE, SODIUM PHOSPHATE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	8-15 (%(w/v))
2	1	1	cacodylate	50 (mM)
3	1	1	calcium acetate	100 (mM)
4	1	2	protein	7-11 (mg/ml)
5	1	2	sodium phosphate	6-25 (mM)