1C3K
CRYSTAL STRUCTURE OF HELIANTHUS TUBEROSUS LECTIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-11 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.927, 104.927, 63.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25500 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 26.900 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.233 |
| Total number of observations | 86459 * | |
| Number of reflections | 11028 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 90.7 | 92.3 * |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 20 * | 26-28% PEG MONOMETHYL ESTER 550, 0.2 M MAGNESIUM ACETATE, pH 7.0, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | EDTA | 3 (mM) | |
| 5 | 1 | drop | P20 | 0.005 (%) | |
| 6 | 1 | reservoir | mPEG550 | 26-28 (%) | |
| 7 | 1 | reservoir | calcium acetate | 0.2 (M) | or magnesium acetate |
| 8 | 1 | reservoir | HEPES | 0.1 (M) |
