1C3G
S. CEREVISIAE HEAT SHOCK PROTEIN 40 SIS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.420, 73.420, 80.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.31100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.367 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.045 | 0.377 * |
| Number of reflections | 6347 | |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 97.0 | 99.5 * |
| Redundancy | 5.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | Sha, B.D., (1999) Acta Crystallogr, D55, 1234. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | phosphate | 10 (mM) | pH7.2 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | phosphate | 100 (mM) | pH8.15 |
| 5 | 1 | reservoir | PEG3350 | 15 (%) |
