1C2B

ELECTROPHORUS ELECTRICUS ACETYLCHOLINESTERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-05-15
Detector	MARRESEARCH
Spacegroup name	F 2 2 2
Unit cell lengths	117.980, 215.870, 229.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 4.500
R-factor	0.369 *
Rwork	0.369
R-free	0.35100
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	4.500
Rmerge	0.200
Total number of observations	101833 *
Number of reflections	7742
Completeness [%]	74.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	20 *	NaKPO4, VAPOR DIFFUSION, pH 8.0, temperature 279K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.4 (M)