1C12

INSIGHT IN ODORANT PERCEPTION: THE CRYSTAL STRUCTURE AND BINDING CHARACTERISTICS OF ANTIBODY FRAGMENTS DIRECTED AGAINST THE MUSK ODORANT TRASEOLIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-11-05
Detector	MARRESEARCH
Spacegroup name	P 2 21 21
Unit cell lengths	40.400, 84.100, 134.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.600
R-factor	0.205
Rwork	0.205
R-free	0.27000
RMSD bond length	0.010
RMSD bond angle	1.650 *
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	0.300
Number of reflections	15749
<I/σ(I)>	12
Completeness [%]	88.0	55
Redundancy	5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	hapten	5 (mM)
2	1	drop	Fab	12 (mg/ml)
3	1	drop	Tris-HCl	100 (mM)
4	1	reservoir	PEG8000	19 (%(w/v))
5	1	reservoir	sodium acetate	75-200 (mM)