1C0U

CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH BM+50.0934

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-10-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	137.300, 108.500, 72.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.520
R-factor	0.224
Rwork	0.232
R-free	0.29800
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.620
High resolution limit [Å]	2.520	2.520
Rmerge	0.087	0.737
Total number of observations	115112 *
Number of reflections	32729
<I/σ(I)>	15.6
Completeness [%]	88.8	64.5
Redundancy	3.5	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5	4 *	see reference 9, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	26 (mg/ml)
2	1	drop	PEG3400	6 (%(w/v))
3	1	drop	citrate/phosphate
4	1	reservoir	PEG	6 (%(w/v))