1C0E

Active Site S19A Mutant of Bovine Heart Phosphotyrosyl Phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-01-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	43.320, 55.470, 131.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.191
Rwork	0.191
R-free	0.24400
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.061	0.306
Number of reflections	15625
<I/σ(I)>	15
Completeness [%]	93.4	67.7
Redundancy	3.0 *	1.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)
2	1	2	PEG4000	30 (%)
3	1	2	Tris	0.1 (M)