1C0B
BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 2.5 DAYS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1996-09-25 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.700, 39.700, 50.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.27200 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MERLOT |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | 2.100 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | |
| Total number of observations | 38053 * | |
| Number of reflections | 5532 | |
| <I/σ(I)> | 33.2 | |
| Completeness [%] | 91.0 | 80 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 4.5 | 295 | 58% methanol (v/v), pH 4.5, SMALL TUBES, temperature 295K |
