1C0A
CRYSTAL STRUCTURE OF THE E. COLI ASPARTYL-TRNA SYNTHETASE : TRNAASP : ASPARTYL-ADENYLATE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.200, 101.200, 231.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.400 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24900 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.324 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | |
| High resolution limit [Å] | 2.400 | |
| Rmerge | 0.059 | 0.214 * |
| Number of reflections | 46618 | |
| Completeness [%] | 98.1 | 93.2 * |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | AMMONIUM SULFATE, GLYCEROL, BIS-TRIS-PROPANE, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | GLYCEROL | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 4 | 1 | 2 | (NH4)2SO4 | ||
| 5 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | tRNAAsp | 4.7 (mg/ml) | |
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | drop | EDTA | 0.25 (mM) | |
| 6 | 1 | drop | sodium cacodylate | 5 (mM) | |
| 7 | 1 | reservoir | glycerol | 15 (%) | |
| 8 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 9 | 1 | reservoir | Bis-Tris-propane-HCl | 50 (mM) |
