1C04
IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.000, 301.400, 576.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 5.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.000 | 3.810 |
| High resolution limit [Å] | 3.750 | 3.750 |
| Rmerge | 0.103 | 0.513 |
| Total number of observations | 957850 * | |
| Number of reflections | 185190 * | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 98.6 | 96 |
| Redundancy | 5.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 * | 19 * | von Bohlen, K., (1991) J. Mol. Biol., 222, 11. * |
| 1 | Vapor diffusion * | 5.6 * | 19 * | von Bohlen, K., (1991) J. Mol. Biol., 222, 11. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCL | ||
| 2 | 1 | 1 | NH4CL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SODIUM ACETATE | ||
| 5 | 1 | 1 | PEG 6000 | ||
| 6 | 1 | 2 | PEG 6000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | 1.2 (M) | ||
| 3 | 1 | drop | 0.005 (M) | ||
| 4 | 1 | drop | PEG6000 | 5-6 (%) | |
| 5 | 1 | drop | acetate | 0.1 (M) | |
| 6 | 1 | drop | 0.00185 (mM) | ||
| 7 | 1 | drop | beta-octylglucoside | 0.0074 (mM) | |
| 8 | 1 | reservoir | 1.7 (M) |
