1BZW

PEANUT LECTIN COMPLEXED WITH C-LACTOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	129.121, 126.748, 76.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.188
Rwork	0.188
R-free	0.22700
Structure solution method	OTHER
Starting model (for MR)	PDB2PEL WITHOUT SUGAR AND WATER
RMSD bond length	0.008
RMSD bond angle	1.600
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.129	0.461
Total number of observations	124179 *
Number of reflections	33099
<I/σ(I)>	14.2	2.33
Completeness [%]	93.8	93.8
Redundancy	3.75	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		HANGING DROP OF 5 MG/ML PROTEIN IN 0.05 M SODIUM PHOSPHATE BUFFER,PH 7.0, CONTAINING 0.2M SODIUM CHLORIDE, 0.02 % SODIUM AZIDE, 10MM C-LACTOSE AND 12% (W/V) PEG 8000 IN THE SAME BUFFER, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
10	1	reservoir	sodium azide	0.02 (%)
11	1	reservoir	C-lactose	10 (mM)
2	1	drop	sodium phosphate	0.05 (M)
3	1	drop		0.2 (M)
4	1	drop	sodium azide	0.02 (%)
5	1	drop	C-lactose	10 (mM)
6	1	drop	PEG8000	12 (%(w/v))
7	1	reservoir	PEG8000	40 (%(w/v))
8	1	reservoir	sodium phosphate	0.05 (M)
9	1	reservoir		0.2 (M)