1BZQ

COMPLEX OF A DROMEDARY SINGLE-DOMAIN VHH ANTIBODY FRAGMENT WITH RNASE A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	66.520, 66.820, 69.340
Unit cell angles	91.43, 117.34, 97.33

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.222
R-free	0.28500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1RUV AND PDB ENTRY 1MEL
RMSD bond length	0.006 *
RMSD bond angle	0.025 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.111 *	0.336 *
Number of reflections	25413
<I/σ(I)>	8.5	3.9
Completeness [%]	99.2	98.2
Redundancy	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4		HANGING DROP METHOD. 5 MG/ML OF THE COMPLEX RESERVOIR SOLUTION IS 0.1 M POTASSIUM PHOSPHATE PH 6.4 15 TO 20% W/V PEG 8000, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	15-20 (%(w/v))
3	1	reservoir	potassium phosphate	0.1 (M)