1BZ9
CRYSTAL STRUCTURE OF MURINE CLASS I MHC H2-DB COMPLEXED WITH A SYNTHETIC PEPTIDE P1027
Replaces: 1A9DExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.350, 109.190, 57.780 |
| Unit cell angles | 90.00, 122.81, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.266 |
| Rwork | 0.252 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hoc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.550 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE (IN CCP4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.091 | 0.292 |
| Total number of observations | 62278 * | |
| Number of reflections | 11820 | |
| <I/σ(I)> | 20.4 | 5.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 25 (%) | |
| 2 | 1 | reservoir | 150 (mM) | ||
| 3 | 1 | reservoir | Gly | 0.8 (M) | |
| 4 | 1 | reservoir | DMSO | 6 (%) | |
| 5 | 1 | reservoir | MES | 25 (mM) | |
| 6 | 1 | drop | protein | 10 (mg/ml) | |
| 7 | 1 | drop | Gly | 0.8 (M) | |
| 8 | 1 | drop | MES | 25 (mM) |
