1BYU

CANINE GDP-RAN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-29
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	60.790, 60.433, 61.200
Unit cell angles	90.00, 101.67, 90.00

Refinement procedure

Resolution	6.000 - 2.150
Rwork	0.176
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SCHEFFZEK ET AL
RMSD bond length	0.010
RMSD bond angle	0.030
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.300	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.030	0.097
Total number of observations	45779 *
Number of reflections	22596
<I/σ(I)>	20.9	7.6
Completeness [%]	94.7	92.2
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *	SEE PUBLICATION, pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG1000	20-35 (%(v/v))
3	1	reservoir		20 (mM)
4	1	reservoir	Tris-HCl	50 (mM)