1BY7

HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-2. LOOP (66-98) DELETION MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-06-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	138.820, 41.730, 77.400
Unit cell angles	90.00, 114.88, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.202 *
Rwork	0.202
R-free	0.26800
Structure solution method	MIR
RMSD bond length	0.017
RMSD bond angle	0.040
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.055	0.350
Total number of observations	176011 *
Number of reflections	27274
Completeness [%]	99.0	90.5 *
Redundancy	6.4	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	PEG8000	15 (%)
3	1	reservoir	magnesium acetate	0.12 (M)
4	1	reservoir	HEPES	0.1 (M)	pH7.5