1BXB
XYLOSE ISOMERASE FROM THERMUS THERMOPHILUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Detector | FUJI |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.340, 144.050, 155.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
R-factor | 0.185 * |
Rwork | 0.180 |
R-free | 0.23200 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 23.850 * |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.078 |
Number of reflections | 66746 |
Completeness [%] | 78.0 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 * | pH 8.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 50 (mM) |