1BX4
STRUCTURE OF HUMAN ADENOSINE KINASE AT 1.50 ANGSTROMS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-08-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.300, 111.080, 49.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.22600 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 23.100 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | SnB |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.048 | 0.185 |
Total number of observations | 259153 * | |
Number of reflections | 57595 | |
<I/σ(I)> | 6.3 | 3.7 |
Completeness [%] | 98.3 | 91.4 |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 20%PEG 4K, 0.16M MGCL2, 0.1M TRIS, PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 20 (%) | |
3 | 1 | reservoir | 0.16 (M) | ||
4 | 1 | reservoir | Tris | 0.1 (M) | can be replaced by HEPES |