1BWZ
DIAMINOPIMELATE EPIMERASE FROM HEMOPHILUS INFLUENZAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 288 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.100, 115.400, 66.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.790 - 2.720 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.24200 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 28.590 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.790 | 2.830 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.060 * | 0.145 * |
| Total number of observations | 33313 * | |
| Number of reflections | 9691 | |
| <I/σ(I)> | 11.61 | 10.89 |
| Completeness [%] | 89.4 | 51.6 |
| Redundancy | 3.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 15 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 25 (mM) | pH7.5 |
| 3 | 1 | drop | 0.05 (%) | ||
| 4 | 1 | reservoir | sodium acetate | 1 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.8 |
