1BWR

PROBING THE SUBSTRATE SPECIFICITY OF THE INTRACELLULAR BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	81.056, 81.056, 72.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.202 *
Rwork	0.205
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PAF ACETYLHYDROLASE
RMSD bond length	0.010
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.400	2.300
Number of reflections	10257
<I/σ(I)>	18.3
Completeness [%]	92.0	87.6
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.8		pH 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-9 (mg/ml)
2	1	reservoir	ammonium sulfate	14 (%)
3	1	reservoir	Tris-HCl