1BW0

CRYSTAL STRUCTURE OF TYROSINE AMINOTRANSFERASE FROM TRYPANOSOMA CRUZI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	285
Detector technology	AREA DETECTOR
Collection date	1996-06-15
Detector	SIEMENS X1000
Spacegroup name	P 1 21 1
Unit cell lengths	61.600, 102.200, 77.900
Unit cell angles	90.00, 110.30, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.157 *
Rwork	0.157
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1art
RMSD bond length	0.006
RMSD bond angle	0.023
Data reduction software	X-GEN
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.051	0.096
Number of reflections	24668
<I/σ(I)>	17	6
Completeness [%]	78.4	47
Redundancy	1.7	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7	285 *	DIALYSIS OF 2.6 MG/ML PROTEIN AGAINST 25 % (W/W) PEG 8000, 5 MM PLP, 0.1 M PHOSPHATE/CITRATE, PH 7.0, 285 K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	25 (%(w/w))
2	1	1	PLP	5 (mM)
3	1	1	citrate/phosphate	0.1 (M)