1BUI

Structure of the ternary microplasmin-staphylokinase-microplasmin complex: a proteinase-cofactor-substrate complex in action

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	190
Detector technology	CCD
Collection date	1998-05-15
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	76.140, 76.140, 324.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.650
R-factor	0.204
Rwork	0.204
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1FAX AND 2SAK
RMSD bond length	0.010
RMSD bond angle	1.814
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.650
Rmerge	0.075
Total number of observations	483198 *
Number of reflections	28745
Completeness [%]	99.5	98 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium HEPES	0.1 (M)
2	1	reservoir	sodium potassium phosphate	0.8 (M)