1BUH

CRYSTAL STRUCTURE OF THE HUMAN CDK2 KINASE COMPLEX WITH CELL CYCLE-REGULATORY PROTEIN CKSHS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-12-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	56.800, 69.950, 60.530
Unit cell angles	90.00, 114.50, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.19
Rwork	0.190
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PERSONAL COMMUNICATION
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Rmerge	0.100
Total number of observations	84909 *
Number of reflections	13023 *
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	20 *	17-19% MEPEG 5K, 100 MM TRIS-HCL PH 7.5, 100 MM KCL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	reservoir	MEPEG5000	17-19 (%)
3	1	reservoir	Tris	100 (mM)
4	1	reservoir		100 (mM)