1BTN

STRUCTURE OF THE BINDING SITE FOR INOSITOL PHOSPHATES IN A PH DOMAIN

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-02
Detector	MARRESEARCH
Spacegroup name	P 4 21 2
Unit cell lengths	69.000, 69.000, 50.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.205
Rwork	0.205
R-free	0.28600
RMSD bond length	0.011
RMSD bond angle	25.620 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	1000.000
High resolution limit [Å]	2.000
Rmerge	0.047
Total number of observations	41713 *
Number of reflections	7830
Completeness [%]	89.6
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium phosphate	10 (mM)
3	1	drop	Ins(1,4,5)P3		3-fold excess
4	1	reservoir	PEG6000	10-15 (%(w/v))
5	1	reservoir	ethanol	30 (%(v/v))
6	1	reservoir	sodium acetate	100 (mM)