1BTM
TRIOSEPHOSPHATE ISOMERASE (TIM) COMPLEXED WITH 2-PHOSPHOGLYCOLIC ACID
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.610, 108.420, 71.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.800 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.300 * |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.091 | |
| Total number of observations | 64784 * | |
| Number of reflections | 16996 * | |
| Completeness [%] | 89.5 | 76.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 10 | 1 | reservoir | EDTA | 1 (mM) | |
| 11 | 1 | reservoir | sodium azide | 1 (mM) | |
| 2 | 1 | drop | MES | 5 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | sodium azide | 1 (mM) | |
| 5 | 1 | drop | 2-phosphoglycolic acid | 2 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 7 | 1 | reservoir | acetate | 100 (mM) | |
| 8 | 1 | reservoir | isopropanol | 8 (%) | |
| 9 | 1 | reservoir | dithiothreitol | 2 (mM) |
