1BTJ

HUMAN SERUM TRANSFERRIN, RECOMBINANT N-TERMINAL LOBE, APO FORM, CRYSTAL FORM 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-05-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	63.000, 77.300, 72.600
Unit cell angles	90.00, 104.30, 90.00

Refinement procedure

Resolution	6.000 - 3.200
R-factor	0.217
Rwork	0.217
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HUMAN TRANSFERRIN N-LOBE HOLO FORM INDIVIDUAL DOMAINS
RMSD bond length	0.013
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.500
High resolution limit [Å]	3.200	3.200
Rmerge	0.111	0.257
Number of reflections	11269
<I/σ(I)>	5.9	2.6
Completeness [%]	99.7	99.9
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	4 *	PROTEIN DROP: 20 MG/ML PROTEIN, 20MM NAHCO3, 50 MM KCL. RESERVOIR: 50 MM POTASSIUM ACETATE, PH 5.3, 35% MPD., VAPOR DIFFUSION, SITTING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		20 (mM)
3	1	drop		50 (mM)
4	1	reservoir	potassium acetate	50 (mM)
5	1	reservoir	MPD	35 (%(v/v))