1BT0

STRUCTURE OF UBIQUITIN-LIKE PROTEIN, RUB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1997-08-15
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	28.600, 35.600, 37.900
Unit cell angles	90.00, 102.80, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.18 *
R-free	0.24600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UBI WITH NON-CONSERVED RESIDUES TRUNCATED INTO ALANINE
RMSD bond length	0.015
RMSD bond angle	16.000 *
Data reduction software	XDS
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700
Rmerge	0.051
Total number of observations	23228 *
Number of reflections	7919
Completeness [%]	95.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	9.0 (mg/ml)
2	1	1	PEG8000	18-20 (%(w/v))
3	1	1	zinc acetate	200-400 (mM)
4	1	1	MPD	2-4 (%)
5	1	1	MES	100 (mM)