1BSR

BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 2 21 21
Unit cell lengths	36.500, 66.700, 107.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.900
R-factor	0.177
Rwork	0.177
RMSD bond length	0.020
RMSD bond angle	3.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.067 *
Total number of observations	59869 *
Number of reflections	17217 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme solution	15-20 (mg/ml)
2	1	1	ammonium sulphate	80 (%sat)
3	1	1	ammonium phosphate	0.1 (mM)
4	1	1	acetate	10 (mM)