1BS2
YEAST ARGINYL-TRNA SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.374, 100.374, 204.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.750 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.26900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 20.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (0.1-0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.042 * | 0.180 * |
| Total number of observations | 168917 * | |
| Number of reflections | 27890 | 2660 * |
| <I/σ(I)> | 35 | 7.3 |
| Completeness [%] | 99.4 | 80 * |
| Redundancy | 6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 17 * | AMMONIUM SULFATE 2.45 M, 100MM TRIS-HCL, PH 7.0 AT 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.45 (M) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | protein | 13.5 (mg/ml) | |
| 4 | 1 | drop | L-arginine | 5 (mM) | |
| 5 | 1 | drop | ATP | 5 (mM) | |
| 6 | 1 | drop | 10 (mM) | ||
| 7 | 1 | drop | Tris-HCl | 50 (mM) |
