1BQC

BETA-MANNANASE FROM THERMOMONOSPORA FUSCA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-03-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	43.702, 46.058, 132.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 1.500
R-factor	0.119 *
Rwork	0.119
R-free	0.17600
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	0.022
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.062	0.331
Number of reflections	42667
<I/σ(I)>	17.2	2.9
Completeness [%]	97.2	92.2
Redundancy	3.22	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		1.85M AMMONIUM SULFATE, 0.1M HEPES, PH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.85 (M)
2	1	reservoir	HEPES	0.1 (M)	pH7.0