1BPO
CLATHRIN HEAVY-CHAIN TERMINAL DOMAIN AND LINKER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 205.825, 205.825, 87.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.29200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 2.600 | |
| Rmerge | 0.052 * | 0.261 * |
| Number of reflections | 62848 * | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 96.0 * | 70 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 21-23 (%) | |
| 2 | 1 | reservoir | 400-600 (mM) | ||
| 3 | 1 | reservoir | PIPES | 100 (mM) | pH6.5 |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | IPTG | 0.1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 5 (mM) |
