1BOI

N-TERMINALLY TRUNCATED RHODANESE

Experimental procedure

Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1997-10
Detector	SIEMENS HI-STAR
Spacegroup name	P 21 21 2
Unit cell lengths	90.690, 73.250, 39.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.000 - 2.200
R-factor	0.162
Rwork	0.162
R-free	0.21900
RMSD bond length	0.012
RMSD bond angle	24.300 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.104	0.190
Total number of observations	109730 *
Number of reflections	13716 *
Completeness [%]	97.1	77.5
Redundancy	8.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.3	4 *	pH 7.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	PEG6000	10 (%(w/v))
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	sodium acetate	50 (mM)
5	1	drop	sodium thiosulfate	1 (mM)
6	1	reservoir	PEG6000	16 (%(w/v))
7	1	reservoir	Tris-HCl	50 (mM)
8	1	reservoir	sodium acetate	50 (mM)
9	1	reservoir	sodium thiosulfate	1 (mM)