1BM8

DNA-BINDING DOMAIN OF MBP1

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1996-06
Detector	RIGAKU RAXIS IIC
Wavelength(s)	0.96, 1.54
Spacegroup name	P 41 21 2
Unit cell lengths	43.350, 43.350, 124.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.710
R-factor	0.197
Rwork	0.197
R-free	0.26100
RMSD bond length	0.009
RMSD bond angle	23.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.780
High resolution limit [Å]	1.700	1.710
Rmerge	0.052
Total number of observations	68232 *
Number of reflections	12811
<I/σ(I)>	11.4
Completeness [%]	93.6	34
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	16 *	drop contains equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		300 (mM)
3	1	drop	sodium phosphate	50 (mM)
4	1	reservoir	sodium citrate	70 (%sat)
5	1	reservoir	imidazole	100 (mM)