1BLI

BACILLUS LICHENIFORMIS ALPHA-AMYLASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	1997-05
Detector	MARRESEARCH
Spacegroup name	P 61
Unit cell lengths	91.300, 91.300, 137.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.154
Rwork	0.154
R-free	0.18500
Structure solution method	PATTERSON SEARCH
Starting model (for MR)	1bpl
RMSD bond length	0.013
RMSD bond angle	25.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.500	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.084	0.418
Number of reflections	46801
<I/σ(I)>	23	2.3
Completeness [%]	91.5	82.9
Redundancy	6.7	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	20 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	HEPES	50 (mM)
4	1	reservoir	PEG500	1 (%(v/v))