1BL5
ISOCITRATE DEHYDROGENASE FROM E. COLI SINGLE TURNOVER LAUE STRUCTURE OF RATE-LIMITED PRODUCT COMPLEX, 10 MSEC TIME RESOLUTION
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 274 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-01 |
| Detector | FUJI |
| Wavelength(s) | 0.7, 1.7 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 106.100, 106.100, 151.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.27100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.800 |
| Data reduction software | LaueView |
| Data scaling software | LaueView |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.500 | 2.400 |
| Rmerge | 0.102 | 0.237 |
| Total number of observations | 360784 * | |
| Number of reflections | 21751 | |
| <I/σ(I)> | 17.2 | 7.5 |
| Completeness [%] | 90.8 | 83.8 |
| Redundancy | 12.9 | 16.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.4 * | Hurley, J.H., (1989) Proc.Nat.Acad.Sci.USA, 86, 8635. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 28 (mg/ml) | |
| 2 | 1 | 1 | ammonium sulfate | 34 (%sat) | |
| 3 | 1 | 1 | 100 (mM) | ||
| 4 | 1 | 1 | 35 (mM) | ||
| 5 | 1 | 1 | citric acid | 9 (mM) | |
| 6 | 1 | 1 | dithiothreitol | 0.2 (mM) |
