1BL0
MULTIPLE ANTIBIOTIC RESISTANCE PROTEIN (MARA)/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 96 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-01 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.100, 47.100, 298.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.30300 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 21.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.067 * | 0.770 * |
| Total number of observations | 121112 * | |
| Number of reflections | 16074 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | micro seeding * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium CACODYLATE | ||
| 2 | 1 | 1 | calcium ACETATE | ||
| 3 | 1 | 1 | PEG 8000 | ||
| 4 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 12 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | calcium acetate | 100 (mM) |
