1BK7

RIBONUCLEASE MC1 FROM THE SEEDS OF BITTER GOURD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1996-02-15
Detector	MACSCIENCE
Spacegroup name	P 21 21 21
Unit cell lengths	38.800, 67.970, 75.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 1.750
R-factor	0.192
Rwork	0.192
R-free	0.24100
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.950
High resolution limit [Å]	1.750	1.750
Rmerge	0.055	0.199
Total number of observations	69704 *
Number of reflections	20323 *
<I/σ(I)>	9.1	3.5
Completeness [%]	98.4	98.2
Redundancy	3.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	6.7		De, A., (1991) J. Mol. Biol., 228, 1271. *