1BK5

KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	1998-02
Detector	BRANDEIS
Spacegroup name	P 21 21 21
Unit cell lengths	157.980, 74.140, 84.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 2.200
R-factor	0.237
Rwork	0.237
R-free	0.27600
Structure solution method	MIR (MULTIPLE ISOMORPHOUS REPLACEMENT)
RMSD bond length	0.007
RMSD bond angle	17.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (0.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.065 *	0.299 *
Total number of observations	525332 *
Number of reflections	48255
<I/σ(I)>	13.6	2.8
Completeness [%]	95.0	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	18-20 (%)
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	HEPES	100 (mM)
4	1	reservoir		20 (mM)