1BK4
CRYSTAL STRUCTURE OF RABBIT LIVER FRUCTOSE-1,6-BISPHOSPHATASE AT 2.3 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 285 |
Detector technology | AREA DETECTOR |
Collection date | 1990-01-15 |
Detector | UCSD MARK III |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 73.790, 80.050, 131.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.177 |
Rwork | 0.177 |
Structure solution method | MIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.700 |
Data reduction software | UCSD-system |
Data scaling software | UCSD-system |
Phasing software | PHASES |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.057 |
Total number of observations | 67887 * |
Number of reflections | 16921 |
<I/σ(I)> | 12.8 |
Completeness [%] | 93.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 | 10-15% PEG 4000, 50-200 MM A.S. IN 25 MM TRIS, pH 7.4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 10-15 (%) | |
2 | 1 | reservoir | Tris-HCl | 25 (mM) | pH7.4 |
3 | 1 | drop | protein | 7-8 (mg/ml) | |
4 | 1 | drop | ammonium sulfate | 50-200 (mM) |