1BJA

ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-06
Detector	RIGAKU
Spacegroup name	P 32 2 1
Unit cell lengths	46.700, 46.700, 139.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.190
R-factor	0.208
Rwork	0.208
R-free	0.29100
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.300
High resolution limit [Å]	2.190	2.190
Rmerge	0.054 *	0.081
Total number of observations	66237 *
Number of reflections	9394
<I/σ(I)>	11	2.5
Completeness [%]	98.5	80
Redundancy	7	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	22 *	Finnin, M.S., (1993) J. Mol. Biol., 232, 301. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	60 (%sat)