1BIS
HIV-1 INTEGRASE CORE DOMAIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11 |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.090, 45.080, 49.430 |
| Unit cell angles | 68.82, 64.74, 62.63 |
Refinement procedure
| Resolution | ? - 1.950 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.301 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.020 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.028 | 0.106 |
| Number of reflections | 20883 | |
| <I/σ(I)> | 25 | 8.6 |
| Completeness [%] | 93.8 | 83.8 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | PROTEIN WAS CRYSTALLIZED FROM 30% PEG 4000, 100 MM HEPES, PH 7.0, 5 MM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.5 (M) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 30 (%) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
