1BIK
X-RAY STRUCTURE OF BIKUNIN FROM THE HUMAN INTER-ALPHA-INHIBITOR COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.300, 55.300, 146.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | C-TERMINAL DOMAIN OF PDB ENTRY 1KNT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.210 |
| Total number of observations | 55165 * | |
| Number of reflections | 8519 | |
| <I/σ(I)> | 13.4 | 10.1 |
| Completeness [%] | 97.7 | 97.6 |
| Redundancy | 6.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2 (M) | |
| 3 | 1 | drop | PEG400 | 4 (%) | |
| 4 | 1 | drop | HEPES | 0.1 (M) |
