1BII
THE CRYSTAL STRUCTURE OF H-2DD MHC CLASS I IN COMPLEX WITH THE HIV-1 DERIVED PEPTIDE P18-110
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Collection date | 1997-10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.260, 92.530, 108.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.400 |
| R-factor | 0.293 * |
| Rwork | 0.278 |
| R-free | 0.33800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vac |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 * | 0.298 * |
| Total number of observations | 199263 * | |
| Number of reflections | 20963 | |
| <I/σ(I)> | 34 | 4.7 |
| Completeness [%] | 96.5 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | Achour, A., (1998) Acta Cryst., D55, 260. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | PEG8000 | 30 (%) |
