1BID
E. COLI THYMIDYLATE SYNTHASE COMPLEXED WITH DUMP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | RIGAKU |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 133.060, 133.060, 133.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.21700 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1tjs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.067 | 0.319 |
| Total number of observations | 92187 * | |
| Number of reflections | 23029 | |
| <I/σ(I)> | 17.2 | 3.4 |
| Completeness [%] | 96.9 | 91.5 |
| Redundancy | 7.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | CRYSTALLIZATION EXPERIMENTS WERE CONDUCTED IN HANGING DROPS CONTAINING 4.2 MG/ML E. COLI TS, 0.38 MM DUMP, 3.8 MM DTT, AND 1.2 M (NH4)2SO4, AT PH 7.8 (20 MM KPO4) SUSPENDED OVER A WELL SOLUTION CONTAINING 2.4 M (NH4)2SO4 AND 1.0 MM DTT., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.2 (mg/ml) | |
| 2 | 1 | drop | dUMP | 0.38 (mM) | |
| 3 | 1 | drop | dithiothreitol | 3.8 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 1.2 (M) | |
| 5 | 1 | drop | potassium phosphate | 20 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 7 | 1 | reservoir | dithiothreitol | 1.0 (mM) |
