1BI9
RETINAL DEHYDROGENASE TYPE TWO WITH NAD BOUND
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 149.800, 167.300, 107.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.500 | 2.700 |
| Rmerge | 0.075 | 0.412 |
| Total number of observations | 864923 * | |
| Number of reflections | 75572 | |
| <I/σ(I)> | 22 | 3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 * | 25 * | pH 7.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | dioxane | 5 (%) | |
| 11 | 1 | reservoir | ethylene glycol | 9-12 (%) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 6 | 1 | drop | NAD | 2 (mM) | |
| 7 | 1 | drop | beta-ionone | 2 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 1.0-1.3 (M) | |
| 9 | 1 | reservoir | MES | 0.1 (M) |
