1BHT

NK1 FRAGMENT OF HUMAN HEPATOCYTE GROWTH FACTOR

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1994-01
Detector	FUJI
Spacegroup name	P 43 21 2
Unit cell lengths	88.320, 88.320, 117.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.196
Rwork	0.196
R-free	0.24700
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	2.051
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.066	0.170
Number of reflections	1807
<I/σ(I)>	11.7
Completeness [%]	94.0	87
Redundancy	5.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		40% PEG 3400, 0.1M HEPES PH 7.5, 0.3M AMMONIUM SULFATE. PROTEIN CONCENTRATION OF 3MG/ML

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	PEG3400	20 (%)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	ammonium sulfate	0.3 (M)