1BHM
RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.800, 81.900, 68.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.189 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.800 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.118 * |
| Total number of observations | 103881 * |
| Number of reflections | 30078 |
| Completeness [%] | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | Strzelecka, T., (1994) J. Mol. Biol, 239, 430. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | WATER | ||
| 11 | 1 | 2 | PEG 8000 | ||
| 12 | 1 | 2 | GLYCEROL | ||
| 13 | 1 | 2 | KCL | ||
| 2 | 1 | 1 | GLYCEROL | ||
| 3 | 1 | 1 | PEG 8000 | ||
| 4 | 1 | 1 | K PHOSPHATE | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | KCL | ||
| 7 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 8 | 1 | 1 | EDTA | ||
| 9 | 1 | 1 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | BamHI | 0.23 (mM) | |
| 10 | 1 | drop | PEG8000 | 12 (%) | |
| 11 | 1 | drop | glycerol | 2 (%) | |
| 12 | 1 | reservoir | PEG8000 | 12 (%) | |
| 13 | 1 | reservoir | 0.15 (M) | ||
| 14 | 1 | reservoir | glycerol | 5 (%) | |
| 2 | 1 | drop | DNA | 0.46 (mM) | |
| 3 | 1 | drop | glycerol | 3 (%) | |
| 4 | 1 | drop | 6.1 (mM) | ||
| 5 | 1 | drop | 0.3 (mM) | ||
| 6 | 1 | drop | 0.15 (M) | ||
| 7 | 1 | drop | Tris-HCl | 3.9 (mM) | |
| 8 | 1 | drop | 0.4 (mM) | ||
| 9 | 1 | drop | 3.9 (mM) |
